Project Description

Hair Bundle Properties

What are the intrinsic properties of hair bundles that dictate their output? How does hair bundle cohesiveness shape the hair cell receptor potential?

Intrinsic hair bundles shape force transfer to the mechanotransduction channel such that the type of stimulus used results in a very different output. The consequences of these output are critical in that they directly shape the hair cell receptor potential. We are characterizing the mechanical properties of both IHC and OHC hair bundles to identify the most relevant mechanical properties as well as the molecular machinery creating these properties. To address these questions, we are using specially designed piezoelectric devices and fluid jet systems for mechanically stimulating the hair bundle why measuring hair bundle movements with high speed cameras or high speed confocal images that monitor calcium entry through the mechanically gated channels. We simultaneously monitor the MET currents.