Calcium (Ca2+) imaging
How many hair cells are activated with a pure tone? How do neighbor hair cells act? What changes does loud sound make?
Sound induces Ca2+ influx into inner hair cells through L-type voltage-gated Ca2+ channels, leading to the release of neurotransmitter. To study sound-evoked Ca2+ responses in the cochlea, we use the GECI GCaMP6 expressed selectively in hair cells. Our goal of this study is to determine dynamics of peripheral auditory encoding by functional monitoring of multiple cochlear cells